EIF1

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Wikipedia

EIF1
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesEIF1, A121, EIF-1, ISO1, SUI1, eukaryotic translation initiation factor 1, Eukaryotic initiation factor 1
External IDsMGI: 105125; HomoloGene: 130538; GeneCards: EIF1; OMA:EIF1 - orthologs
Orthologs
SpeciesHumanMouse
Entrez

10209

20918

Ensembl

ENSG00000173812

ENSMUSG00000035530

UniProt

P41567

P48024

RefSeq (mRNA)

NM_005801

NM_011508

RefSeq (protein)

NP_005792

NP_035638

Location (UCSC)Chr 17: 41.69 – 41.69 MbChr 11: 100.21 – 100.21 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Eukaryotic translation initiation factor 1 (eIF1) is a protein that in humans is encoded by the EIF1 gene. It is related to yeast SUI1.[5][6][7]

eIF1 interacts with the eukaryotic small (40S) ribosomal subunit and eIF3, and is a component of the 43S preinitiation complex (PIC).[8] eIF1 and eIF1A bind cooperatively to the 40S to stabilize an "open" conformation of the preinitiation complex (PIC) during eukaryotic translation initiation.[8] eIF1 binds to a region near the ribosomal P-site in the 40S subunit and functions in a manner similar to the structurally related bacterial counterpart IF3.[9]

Structure

eIF1's structure was first determined in 1999 by solution-state NMR spectroscopy, which revealed that it consists of a five-stranded beta-sheet which is sided by two alpha-helices.[10] Crystallographic experiments showed that eIF1 is located at the P-site of the small ribosomal subunit, binding to the 18S rRNA with a basic surface.[11] To date, a number of cryo-EM structures have been solved that include eIF1 in the context of various translation initiation complexes.[12][13][14]

Function

In eukaryotic cells, translation initiation on an mRNA involves scanning of the mRNA by the 43S pre-initiation complex in search of the translation initiation start codon.[15] Accurate identification of the start codon is very important, as other translation start sites may lead to the production of defect proteins. A codon is detected as a start codon by interaction with the tRNA's anticodon that is positioned in the P-site of the small ribosomal subunit, which leads to closing of the pre-initiation complex.[16] eIF1 is positioned on the small ribosomal subunit such that it blocks the closure of the pre-initiation complex. It thereby aids in selecting the correct start codon, since only the correct codon-anticodon interaction provides enough energy to displace eIF1 and thus close the pre-initiation complex.[17]

See also

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000173812 – Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000035530 – Ensembl, May 2017
  3. ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ↑ Fields C, Adams MD (January 1994). "Expressed sequence tags identify a human isolog of the suil translation initiation factor". Biochemical and Biophysical Research Communications. 198 (1): 288–91. Bibcode:1994BBRC..198..288F. doi:10.1006/bbrc.1994.1040. PMID 7904817.
  6. ↑ Sheikh MS, Fernandez-Salas E, Yu M, Hussain A, Dinman JD, Peltz SW, Huang Y, Fornace AJ (June 1999). "Cloning and characterization of a human genotoxic and endoplasmic reticulum stress-inducible cDNA that encodes translation initiation factor 1(eIF1(A121/SUI1))". The Journal of Biological Chemistry. 274 (23): 16487–93. doi:10.1074/jbc.274.23.16487. PMID 10347211.
  7. ↑ "Entrez Gene: EIF1 eukaryotic translation initiation factor 1".
  8. 1 2 Aitken CE, Lorsch JR (June 2012). "A mechanistic overview of translation initiation in eukaryotes". Nature Structural & Molecular Biology. 19 (6): 568–76. doi:10.1038/nsmb.2303. PMID 22664984. S2CID 9201095.
  9. ↑ Fraser CS (July 2015). "Quantitative studies of mRNA recruitment to the eukaryotic ribosome". Biochimie. 114: 58–71. doi:10.1016/j.biochi.2015.02.017. PMC 4458453. PMID 25742741.
  10. ↑ Fletcher, C.Mark; Pestova, Tatyana V.; Hellen, Christopher U.T.; Wagner, Gerhard (1999). "Structure and interactions of the translation initiation factor eIF1". The EMBO Journal. pp. 2631–2637. doi:10.1093/emboj/18.9.2631. PMC 1171342. PMID 10228174.
  11. ↑ Rabl, Julius; Leibundgut, Marc; Ataide, Sandro F.; Haag, Andrea; Ban, Nenad (2011). "Crystal Structure of the Eukaryotic 40 S Ribosomal Subunit in Complex with Initiation Factor 1". Science. 331 (6018): 730–736. Bibcode:2011Sci...331..730R. doi:10.1126/science.1198308. hdl:20.500.11850/153130. PMID 21205638.
  12. ↑ Petrychenko, Valentyn; Yi, Sung-Hui; Liedtke, David; Peng, Bee-Zen; Rodnina, Marina V.; Fischer, Niels (2025). "Structural basis for translational control by the human 48S initiation complex". Nature Structural & Molecular Biology. 32: 62–72. doi:10.1038/s41594-024-01378-4.
  13. ↑ Brito Querido, Jailson; Sokabe, Masaaki; Díaz-López, Irene; Gordiyenko, Yuliya; Zuber, Philipp; Du, Yifei; Albacete-Albacete, Lucas; Ramakrishnan, V.; Fraser, Christopher S. (2024). "Human tumor suppressor protein Pdcd4 binds at the mRNA entry channel in the 40S small ribosomal subunit". Nature Communications. 15 (1) 6633. Bibcode:2024NatCo..15.6633B. doi:10.1038/s41467-024-50672-8. PMC 11310195. PMID 39117603.
  14. ↑ Villamayor-Belinchón, Laura; Sharma, Prafful; Gordiyenko, Yuliya; Llácer, Jose L.; Hussain, Tanweer (2024). "Structural basis of AUC codon discrimination during translation initiation in yeast". Nucleic Acids Research. 52 (18): 11317–11335. doi:10.1093/nar/gkae737. PMID 39193907.
  15. ↑ Hinnebusch, Alan G. (2017). "Structural Insights into the Mechanism of Scanning and Start Codon Recognition in Eukaryotic Translation Initiation". Trends in Biochemical Sciences. 42 (8): 589–611. doi:10.1016/j.tibs.2017.03.004. PMID 28442192.
  16. ↑ Hussain, Tanweer; Llácer, Jose L.; Fernández, Israel S.; Munoz, Antonio; Martin-Marcos, Pilar; Savva, Christos G.; Lorsch, Jon R.; Hinnebusch, Alan G.; Ramakrishnan, V. (2014). "Structural Changes Enable Start Codon Recognition by the Eukaryotic Translation Initiation Complex". Cell. 159 (3): 597–607. doi:10.1016/j.cell.2014.10.001. PMC 4217140. PMID 25417110.
  17. ↑ Thakur, Anil; Hinnebusch, Alan G. (2018). "EIF1 Loop 2 interactions with Met-tRNA i control the accuracy of start codon selection by the scanning preinitiation complex". Proceedings of the National Academy of Sciences. 115 (18): E4159 – E4168. Bibcode:2018PNAS..115E4159T. doi:10.1073/pnas.1800938115. PMC 5939108. PMID 29666249.
  18. ↑ "EIF1 - Eukaryotic translation initiation factor 1 - Homo sapiens (Human) - EIF1 gene & protein". www.uniprot.org. Retrieved 2018-09-23.

Further reading